Cell Signaling Technology
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
9106S
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Western blot assays to validate protein array data in ovarian cancer cell lines.
Western Blot
Ovarian cancer cell line panel lysates
1:1000 in 5% BSA in TBS-T, overnight at 4 degrees C, on a rocker
5% BSA in TBS-T
1:5000, anti-Ms in 5% BSA in TBS-T, 30 minutes at room temp, on a rocker
None
HRP Chemiluminescence
Great antibody, very clear bands!
Very good antibody, doesn't require a lot of optimization
Great antibody from CST. Clear bands, quick and easy to use.