Cell Signaling Technology
Phospho-Akt (Ser 473) (D9E) XP® Rabbit mAb
4060S
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Western blot assays to validate protein array data in ovarian cancer cell lines.
Western Blot
Ovarian cancer cell line panel lysates
1:1000 in 5% BSA in TBS-T, overnight at 4 degrees C, on a rocker
5% BSA in TBS-T
1:5000, anti-Rb in 5% BSA in TBS-T, 30 minutes at room temp, on a rocker
None
HRP Chemiluminescence
This is a great antibody for phospho AKt, clear bands with essentially no background!
Very clean bands!
I would highly recommend! Great antibody, no background, and CST has multiple size options to keep it cost-effective.