Santa Cruz Biotechnology
anti-MMP9 (M-17)
sc-6841
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Protein extracts from astrocytic primary cultures treated with two different ER-stressor were blotted and then probed against MMP9 to analyze activation of MMP9 after UPR activation.
Western Blot
Protein extract from cultured astrocytes
1:1000, Over night, 4˚C, incubated in 5% slime milk in TBS-T
5% slime milk in TBS-T
1:4000, 1 hour, room temperature, incubated in 5% slime milk in TBS-T
Not necessary
Chemiluminescent detection
Didn't work as expected, it recognized several bands, one of them at ~90KDa (expected size)
None
It appears an expected protein size
Is not a clear blot
For the final result, it must work a little to 'clean' the image