Sigma
Anti-B-Actin
A5441
Browse Similar Products: B-Actin Western Blot Antibodies
Protein extracts from astrocytic primary cultures and Conditioned medium were analyzed for different secretory proteins. Once obtained the conditioned medium, proteins were bolted and probed against b-Actin to probe that conditioned medium samples were free from cellular debris.
Western Blot
Protein extract of cultured astrocytes and conditioned medium
1:5000, Over night, 4˚C, incubated in 5% slime milk in TBS-T
5% slime milk in TBS-T
1:4000, 1 hour, room temperature, incubated in 5% slime milk in TBS-T
Not necessary
Chemiluminescent detection
It appears a strong reaction in the protein cell extract and nothing in the protein extract form conditioned medium
None
It never fail
Nothing
Is an excellent antibody...