Cell Signaling Technology
Nanog (D2A3) XP® Rabbit mAb (Mouse Specific
8822
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Browse Similar Products: Nanog Western Blot Antibodies
We use nanog as a marker for pluripotency to ensure our mouse ES cells haven't differentiated following different treatments.
Western Blot
Mouse ES cells
1:1000 4C overnight
5% BSA
1:3000 in 5% BSA for one hour at RT
N/A
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Not a great antibody, multiple smeary bands make imaging and analysis difficult.
None
Cheap
Hard to analyze, multiple smeary bands
Wouldn't recommend.