Biorbyt
CWC22 antibody
orb156499
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Browse Similar Products: CWC22 Western Blot Antibodies
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.
Western Blot
Mouse ES cells
1:1000 4C overnight
5% milk
1:3000 in 5% milk for one hour at RT
N/A
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Very high background makes imaging and analysis difficult.
None
Company will refund
High background, multiple bands
Do not recommend.