Novus Biologicals
beta-Actin Antibody
AC-15
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Browse Similar Products: Beta Actin Western Blot Antibodies
Beta actin is a commonly used loading control. It is one of the non-muscular actins which is expressed in all eukaryotic cells. We probed this molecule as an internal loading control.
Western Blot
Whole cell lysates of different cancer cells
diluted 1: 1000 in blocking solution, incubated for overnight at 4 degrees
5% non fat dry milk in TBST
Anti-mouse secondary antibody
None
ECl
Single bands of beta actin were observed ensuring uniform loading in all samples
Single band with no non-specific reactivity
Nothing
It's a nice antibody for western blotting.