Cell signaling
CDK4 (D9G3E) Rabbit mAb
12790
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Cyclin dependent kinases play a vital role in driving cell cycle progression in an efficient and errorproof manner. CDK4/cyclin D3 activity is observed during progression through the G2-phase of the cell cycle. We used the CDK4 antibody to determine the cell cycle arrest in cells.
Western Blot
Whole cell lysates treated with different toxic agents
Diluted 1: 1000 in blocking solution, incubated for overnight at 4 degrees
5% non fat dry milk in TBST
Anti-rabbit secondary antibody
None
ECl
We could detect G2 arrest in cells by determining CDK4 cells in cells.
No non specific bands
Overall, its a nice antibody for cell cycle analysis