Abcam
Anti-beta Actin antibody - Loading Control
ab8229
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Browse Similar Products: Anti-beta Actin Western Blot Antibodies
Beta actin is cytoskeleton protein and is used as a loading control for western blotting. Since it is ubiquitously expressed in all eukaryotic cells, its expression levels are a measure to reflect uniform loading of samples. We used this molecule as an internal loading control.
Western Blot
Whole cell lysates of different cancer cells
Diluted 1:1000 in blocking solution, incubated for overnight at 4 degrees
5% non fat dry milk in TBST
Anti-goat secondary antibody
None
ECl
Single band was obtained by western blotting
Single band with no non-specific reactivity
Nothing
A nice antibody with specific binding to beta actin