Santa Cruz Biotechnology
GAPDH Antibody (FL-335)
sc-25778
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Browse Similar Products: GAPDH Western Blot Antibodies
GAPDH is a commonly used loading control for western blotting and qPCR. Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH) is an important enzyme involved in glycolysis. Because it is a house keeping gene, GAPDH is a commonly used loading control for western blotting and qPCR. We used this antibody as an internal control for western blotting.
Western Blot
Whole cell lysates of different cancer cells
Diluted 1: 1000 in blocking solution, incubated for overnight at 4 degrees
5% non fat dry milk in TBST
Anti-rabbit secondary antibody
None
ECl
Uniform loading was confirmed by GAPDH quantification.
Reproducible results
Nothing
Overall, a good antibody