Rabbit anti-CDCP1 antibody #4115 from Cell Signaling

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

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Company:

Cell Signaling

Product Name:

CDCP1 Antibody #4115

Catalog Number:

4115

Rabbit antibody #4115 from Cell Signaling is an excellent antibody for detection of CDCP1 in human tumor cells. It recognizes both the full-length 135 kDa and the cleaved 70 kDa form of CDCP1.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tumor cell lysate

Primary Incubation

Overnight, at 4-8 degrees Celsius, with rocking, at 1 ug/mL in 5% milk/PBS/0.05% Tween 20

Blocking Agent

5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)

Secondary Incubation

Goat anti-rabbit antibody conjugated with HRP at 1:5,000 in 5% milk/PBS/Tw

Tertiary Incubation

None

Detection

SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific)

Results Summary

The polyclonal antibody #4115 from Cell Signaling is one of the best anti-CDCP1 antibodies. It detects both the full-length 135-kDa CDCP1 protein as well as the cleaved 70-kDa fragment.

Additional Notes

The antibody can be reused multiple times if stored at -20oC after blottings.

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Summary

The Good

The antibody is highly sensitive and specific (does not produce any non-specific bands).

The Bad

None detected

The Bottom Line

I would highly recommend the rabbit antibody #4115 from Cell Signaling for detection of CDCP1 by western blotting.

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