A Decent Antibody

August 01, 2016

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

PCNA (PC10) Mouse mAb

Catalog Number:

2586

PCNA is a proliferating cell marker. We checked PCNA expression in several cells to assess their proliferation abilities.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Different cancer cells

Primary Incubation

1: 1000 in blocking solution for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

Anti mouse HRP for an hour at room temperature

Tertiary Incubation

None

Detection

ECL

Results Summary

We could demonstrate the differential proliferative potential in different cancer cells.

Additional Notes

None

Related Categories

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Summary

The Good

No background

The Bad

none

The Bottom Line

It's a good antibody for western blot

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