Excellent Antibody

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

cytochrome c Antibody (H-104)

Catalog Number:

sc-7159

Apoptosis is crucial for maintaining homeostasis in the system. Apoptosis/cell death can be induced by extrinsic as well as intrinsic factors. Cytochrome C is a part of intrinsic apoptotic pathway and translocates from mitochondria to cytosol in apoptotic cell. We are trying to explore the mechanism by which our drug kills the cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293 T cell lysate

Primary Incubation

1: 500 in blocking solution for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

anti rabbit HRP for an hour

Tertiary Incubation

none

Detection

ECL

Results Summary

We can clearly observed the translocation of cytochrome c from mitochondria to cytosol in apoptotic cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No background

The Bad

none

The Bottom Line

Its a good antibody for routine western blotting.

Share your experience with other scientists. Write a Review! »

Join the discussion