Decent Antibody

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

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Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

p-p21 Antibody

Catalog Number:

sc-20220-R

p21 is a kinase inhibitor which contributes to the regulation of cell division, mediation of negative growth signals, differentiation and apoptosis. It is induced by p53 and regulates cancer cell proliferation and is involved in cell cycle.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Hela cell lysate

Primary Incubation

1: 1000 in blocking solution for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

Anti rabbit HRP for an hour at room temperature

Tertiary Incubation

None

Detection

ECL

Results Summary

Phospho p21 is induced upon stimulation with UV light.

Additional Notes

None

Related Categories

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Summary

The Good

Highly sensitive

The Bad

Gives background in couple of cancer cells

The Bottom Line

Overall, its a good antibody

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