Invitorgen
Goat anti-Mouse IgG Fc Secondary Antibody, HRP conjugate
31437
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This reagent is a highly sensitive secondary antibody. We analyze samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages and bacterial extracts. We use this specific antibody to determine whether the target is expressed in the plant by standard western blotting after incubation with a suitable primary antibody. This antibody works excellent for detection, we tested several comparable products from various companies.
Western Blot
Arabidopsis seedlings or leaves
1 to 1000, overnight
5% milk TBST
1 to 2500
n.a.
ECL
Very clear results from tissue lysates
none
Very good clear signal and reproducibility
great amplification antibody when used with ECL detection