Cell Signaling Technology
Phospho-Akt (Ser473) Antibody
9271
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PhosphoAKT is active form of AKT that drives several cellular functions such as cell proliferation, cell differentiation and cell cycle progression. It is induced in the cell by several growth factors including EGF, IGF etc. It is one of the most extensively studied molecule and we have tried the effect of our drug on its levels.
Western Blot
Hela cell lysate
Overnight at 4 degrees on rocker shaker
5% non fat dry milk in TBST
Anti rabbit HRP for an hour
None
ECL
We treated Hela cells with IGF-1 for different time points. pAKT levels were induced in HEla cells upon treatment.
Reproducible and no non specific background
Little expensive
Overall, its a good antibody for routine use.