Phospho-Cdk Antibody With High Background

August 04, 2016

BlueskyReddit
Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology Inc.

Product Name:

p-Cdc2 p34 Thr 161

Catalog Number:

sc-12341

This antibody directed against phospho-Cdk produces a high background.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Arabidopsis seedlings or leaves

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

n.a.

Detection

ECL

Results Summary

Did not work with secondary antibody from Thermo using pico or femto detection (ECL kit;Thermo). high background, low specificity.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

n.a.

The Bad

Doesn't work

The Bottom Line

Useless, did not get a refund

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