IL-10 Determination Using Flow Cytometry

University of California
Internal Medicine
Assistant Project Scientist

Overall

Quality of Results

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Company:

BD Biosciences

Product Name:

Mouse IL-10 CBA kit

Catalog Number:

558300

IL-10 levels in the serum samples from mice injected with GFP or IL-27 were determined using BD Biosciences CBA kit. The levels produced are low and specific results were desired.

Experimental Design and Results Summary

Application

Flow cytometry/ELISA

Starting Material

Serum samples from various mice injected with control GFP and IL-27

Protocol Overview

Same as provided with the kit

Tips

Beads should be thoroughly mixed before using otherwise negative results can be obtained

Results Summary

Up to 5 Picograms levels can be determined

Additional Notes

None

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Summary

The Good

Low levels pgs can be determined

The Bad

Kit is not cheap

The Bottom Line

Overall good if quick assay is desired to determine the levels from the multiple samples but expensive though.

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