Staining PBMC With Anti-CD45 BUV805

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

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Company:

BD Biosciences

Product Name:

Anti-human CD45 BUV805

Catalog Number:

564914

This antibody was used as a WBC marker in a hematopoietic lineage staining panel for human PBMC. It was chosen because it is a commonly used clone, and BUV805 is a bright fluorochrome with minimal spillover into other detectors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD45 staining was as expected, with nearly all buffy coat cells being positive in the BUV805 channel.

Additional Notes

None

Related Categories

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Summary

The Good

Works well.

The Bad

High cost

The Bottom Line

Will definitely keep using in this panel.

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