Staining PBMC With Anti-CD4 BV785

August 15, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD4 BV785

Catalog Number:

300553

This antibody was used to phenotype CD4+ T cells in human blood. It was chosen because it is a commonly used clone for CD4, BV785 works well in the staining panel, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD4 staining was as expected, with positive cells fully resolved from negative cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will use again.

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