Staining PBMC With Anti-CD3 BV786

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-human CD3 BV786

Catalog Number:

563800

This antibody was used in a T cell phenotype staining panel along with CD56 PE/Cy7 to distinguish T cells from NK and NKT cells in human PBMC. It was chosen because it is a commonly used clone for CD3 and BV786 works well with the other fluorochromes in the panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD3 staining was as expected and T cell populations were fully resolved from NK and NKT cells.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will definitely continue to use this antibody in this staining panel.

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