R&D Systems
Human CXCL8/IL-8 DuoSet ELISA
DY208-05
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Stimulating human cells with bacteria is a common experiment in the lab, even as a simple positive control. The readout is often CXCL8/IL-8. Hence, it is pivotal that labs have a technique to measure it accurately and easily. This kit fits the bill.
ELISA
Media supernatants harvested after co-culturing cells and bacteria
Capture antibody is placed on a plate overnight. Next day, non-specific binding is blocked, and the sample is added. A detection antibody follows. Finally, streptavidin-HRP catalyzes the conversion of color (TMB) which is stopped with a strong acid.
I recommend using R&D's ancillary reagent kit
Results are solid. Stimulated cells produce more CXCL8/IL-8 than controls. A standard curve is used to work out the concentrations in the samples based on their OD.
None
Reliable, reproducible data.
A good ELISA kit for human CXCL8/IL-8 determination.
Product Name: Human CXCL8/IL-8 DuoSet ELISASupplier Page