Staining PBMC With Anti-CD8 Alexa488

August 04, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD8 Alexa488

Catalog Number:

300916

This antibody was used to phenotype T cells in human peripheral blood. It was chosen because it is a commonly used clone, Alexa488 is very bright, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD8 staining was as expected.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will continue to use this antibody in this staining panel.

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