Staining Mouse RBC With Anti-TER-119 BV421

August 03, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse TER-119 BV421

Catalog Number:

116233

This antibody was used as an RBC marker in a hematopoietic lineage staining panel. It was chosen because it is a commonly used clone, BV421 was an open channel in the panel, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Peripheral mouse blood

Primary Incubation

1uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

1:50 dilution of anti-mouse FcBlock and 2% FBS.

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

TER-119 staining was as expected, as 100% of RBC exhibited a strong signal in the BV421 channel as compared to unstained cells.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will continue to use this antibody in this staining panel.

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