Staining PBMC With Anti-CD8 PerCP/Cy5.5

July 25, 2016

BlueskyReddit
Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD8 PerCP/Cy5.5

Catalog Number:

344710

This antibody was used to phenotype CD8+ T cells so that cytokine production could be analyzed. It was chosen because it is a commonly used clone, PerCP/Cy5.5 is a bright fluorochrome, and Biolegend has the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD8 staining was as expected

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will definitely use this again.

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