Abcam
Anti-Histone H3 antibody
ab1791
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Histones are highly alkaline proteins found in eukaryotic cell nuclei that package and order the DNA into structural units called nucleosomes. This group of small proteins consists of the H1 linker histones and the core histones H2A, H2B, H3 and H4.
Immunofluorescence
Primary human neutrophils
1:100
BSA 1%
anti-Rabbit IgG (H+L) Secondary Antibody (Invitrogen). Goat. Polyclonal. Alexa Fluor® 488
None.
CLSM: Blue Exc.: 405nm Em.: 450/50; Green Exc.: 488 nm Em.: 525/50; Red Exc.: 561 Em.: 595/50.
When we studied the histone H3 distribution in human cells by fluorescence staining, we observed an excellent mitochondria staining with very good signal with small background (corrected with lasers) at 1:100 dilution. At 1:200 dilution, the signal was very low. Image. Green (Histone H3); Red (actin cytoskeleton); Blue (DAPI, nuclei). Magnification X1000.
None
Easy to use.
High incubation times may improve signal.