Epitomics
RBBP4 RabMAb
2566-1
Browse Similar Products: Rbbp4 Western Blot Antibodies
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.
Western Blot
Mouse ES cells
1:10000 4C overnight
5% milk
1:3000 in 5% milk for one hour at RT
N/A
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Staining was very faint, but antibody seems to be specific. Need to test different concentrations to optimize staining.
None
Specific
Faint staining at recommended concentration
It's alright, but conditions need to be optimized, which takes time.