Perfect CD25 Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Human CD25 PE-Cyanine7

Catalog Number:

25-0259-42

I am investigating several activation markers on PBMCs after stimulation with various treatments and inhibitors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

30 min, RT, in the dark

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSTII

Results Summary

PBMC need to be stimulated before CD25 is strongly visible. On a small amount of CD4+ it is already visible (Tregs). Antibody works great.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to handle

The Bad

None

The Bottom Line

Perfect anti-CD25 antibody

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