Bethyl
Rabbit anti-COX4 antibody
A301-899A
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The rabbit anti-COX4 antibody A301-899A from Bethyl was used for immunofluorescent staining of human monocyte-derived macrophages. This antibody produced good results consistent with mitochondrial localization of COX4.
Immunofluorescence
Human monocyte-derived macrophages (grown on glass slides, fixed in 1% formaldehyde in PBS, permeabilized with 0.1% saponin)
Overnight, at 4-8 degrees Celsius, at 2 ug/mL in PBS/1% BSA
10% normal donkey serum; 1 hr; RT
Donkey anti-rabbit IgG conjugated with DyLight649 from Biolegend, 1:500, 1 hr
NuncBlue was used to highlight cell nuclei
IF microscope; Olympus
The anti-COX4 rabbit antibody A301-899A from Bethyl produced good staining in human monocyte-derived macrophages permeabilized with saponin. The staining was consistent with the mitochondrial localization of COX4.
None
The antibody worked at a relatively low concentration of 2 ug/mL and produced the expected pattern of staining.
None detected
I would recommend this antibody for detection of COX4 by immunostaining.