IRDye® 800CW Goat Anti rabbit IgG

July 13, 2016

School of Pharmacy
University of Colorado, Denver
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Company:

Li-cor

Product Name:

IRDye® 800CW Secondary Antibodies

Catalog Number:

925-32211

The antibody was used to detect the primary antibody specific retinal protein. It gave very good signal even the protein quantity is less in the rat retina.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat Retina

Primary Incubation

Overnight

Blocking Agent

Dry Milk

Secondary Incubation

2 hrs

Tertiary Incubation

Not applicable

Detection

IR imager

Results Summary

The secondary antibody showed very good signal at lower protein. The sensitivity of this antibody was found to be very good.

Additional Notes

None

Image Gallery

Summary

The Good

Higher sensitivity

The Bad

Needed to add more

The Bottom Line

Can be used to detect the small amount of protein is the antibody quantity is not the issue.

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