Anti-GST Purification Tag Antibody

July 14, 2016

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Mouse Anti-Glutathione S-Transferase (Clone G172-1138)

Catalog Number:

554805

GST affinity purification tag can be easily detected using this antibody and therefore used as loading or interaction control in various experiments such as heterologous protein expression and purification or pull-down studies..

Experimental Design and Results Summary

Applications

Western Blot

Sample

Insect cell culture pellets and lysate

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

n.a.

Detection

tetrazolium

Results Summary

Very clear results from tissue lysates

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good clear signal and reproducibility

The Bad

n.a.

The Bottom Line

Anti-GST purification tag antibody

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