Santa Cruz
Rabbit antibody against the N-terminus of caveolin
sc-894
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The rabbit polyclonal antibody sc-894 from Santa Cruz was used for immunofluorescent detection of caveolin in human prostate cancer cells. In formaldehyde-fixed cells, this antibody produced excellent staining at 1:500 dilution.
Immunofluorescence
Human prostate cancer PC-3 cells, grown on glass coverslips, fixed in 1% formaldehyde for 20 min RT, and permeabilized in 0.2% Triton X-100 for 5 min, RT.
Overnight, at 4-8 degrees Celsius, at 1:500 dilution (0.2 ug/ml)
5% donkey serum in PBS 0.001% Triton X-100
Donkey anti-rabbit IgG conjugated with DyLight488 from Biolegend, 1:500; 1 hr
NuncBlue was used to highlight cell nuclei
IF microscope; Olympus
The antibody produced excellent staining in formaldehyde-fixed and permeabilized prostate cancer cells.
This antibody worked extremely well also in human monocyte-derived macrophages.
The antibody is very sensitive, worked at high dilution (1:500) of the original 0.1 ug/ml stock solution.
None noticed
I would highly recommend this antibody for detection of caveolin by immunofluorescent staining.