Mouse STAT1 Antibody for Western Blot

Medical Microbiology and Immunology
The University of Toledo HSC
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Mouse STAT1 antibody

Catalog Number:

9172S

This antibody was used to test for knockdown at the protein level in fibroblasts and it worked excellently.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast cells

Primary Incubation

1:1000 Overnight

Blocking Agent

5% Milk

Secondary Incubation

1 hour (Goat anti-mouse 1:3000)

Tertiary Incubation

1 hour

Detection

Enhanced chemilluminiscence

Results Summary

This antibody was found to work very well under the conditions tested. There was less protein in the knockdown cells as expected and higher protein expression in the positive controls. This antibody also had cross-species reactivity and this was favorable for my work!

Additional Notes

Photo shows STAT1 bands (top row) and beta-actin control (bottom row)

Related Categories

Image Gallery

Summary

The Good

Easy to optimize, low dilution works

The Bad

Higher price point

The Bottom Line

I would recommend this antibody for western blot in mouse cells.

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