eBioscience
Rat IgG2a K Isotype Control APC
17-4321
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I use flow cytometry to characterize cell populations in murine lungs. My goal is to see how these populations change in response to pathogens. I chose this product because I needed an isotype control for my marker of interest and this was recommended by eBioscience.
Flow Cytometry
Murine lungs
0.5 ug/10^6 cells, 20 minutes on ice
Anti-mouse CD16/32
No secondary
No tertiary
Flow cytometer
When I looked at this control after running it on a flow cytometer, it gave me the results I wanted. It did not bind to anything in my sample, hopefully confirming that my marker of interest was specific and that there was no background binding occurring.
None
Really easy to use and no need for optomization.
I can't think of anything bad about this product.
I liked this isotype and I will continue to use it in further experiments.