Good Antibody for Detecting FAS L Expression

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Fas Ligand antibody

Catalog Number:

ab87023

Unstimulated and PMA/Ionomycin stimulated human PBMCs were blocked with an MMP7 inhibitor and later stained with this antibody. Stained cells were then analysed by flow cytometry

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on FITC Channel

Results Summary

Was able to detect FAS- L expression, which confirm the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Good Specificity

The Bad

None

The Bottom Line

Good antibody to detect FAS-L expression

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