Biolegend
Human IL-15 ELISA MAX™ Deluxe
435104
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I used this ELISA kit to quantify human IL-15 level in cell culture supernatants collected from human IL-15 plasmid transfected cells. Result clearly demonstrated the usefulness of this ELISA kit in quantifying IL-5 level in cell culture supernatants.
ELISA
Human IL-15 plasmid tranfected cell culture supernatants
1. Plates were coated with capture antibody overnight at 4 degree 2. Plates were washed three times and blocked for 1 hr with 1X assay diluent 3. Plates were washed three times and incubated with IL-15 standards and unknown samples for two hours 4. Samples were washed three times and incubated with detection antibody for 1 hr 5. Plates were washed three times and incubated with Avidin HRP for 30 min 6. Plates were washed 4 times and incubated with 100 ul substrate solution for 15 minutes 7. After 15 minutes 100 ul stop solution was added and absorbance was measured at 450.
Follow the protocol in data sheet
Using this ELISA kit I was able to quantify the amount of IL-15 in cell culture supernatants.
None
Reproducibility
Excellent ELISA kit to quantify human IL-15 level in cell culture supernatants and serum
Product Name: Human IL-15 ELISA MAX™ DeluxeSupplier Page