BD Biosciences
PE Mouse Anti-Stat5 (pY694)
612567
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Browse Similar Products: Anti-Stat5 Flow Cytometry Antibodies
Human PBMCs were either left unstimulated or stimulated with 500 U/ml recombinant Human IL-2 for 15 minutes at 37°C. Cells were fixed and then permeabilized in BD Phosflow™ Perm Buffer III. Cells were then washed twice and stained with PE-conjugated phospho-Stat5 antibody. The cells were analyzed on a BD FACS canto II flow cytometer.
Flow Cytometry
Human PBMCs
30 min
None
30
Flow cytometry on PE Channel
The results show a clear p-stat5 positive and negative population, which confirms the specificity of the antibody.
Data is reproducible with this reagent
Need standardization
Very good anti-human p-stat5 antibody