BD Biosciences
APC Rat Anti-Human IL-2
554567
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Human PBMCs were stimulated (6 hours) with PMA and Ionomycin in presence of protein transport inhibitor Golgi Stop. The cells were first stained with anti human CD4 and CD8 antibodies followed by fixation with BD Cytofix™ Buffer. The cells were later permeabilized with BD Perm/Wash™ Buffer and stained with and APC Rat Anti-human Il-2 antibody.
Flow Cytometry
Human PBMCs
30 min
None
30
Flow cytometry on PB and APC channel
Positive staining with IL-2 in PMA/Ionomycin treated group confirm the specificity of this antibody.
Good Specificity
Very good anti-human IL-2 antibody