Very Good Anti-Human CD45 RO Antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Human CD45RO

Catalog Number:

555492

Human PBMCs were isolated from healthy donors and stained with FITC conjugated CD45 RO antibody. Stained cells were analyzed in a BD FACS Canto II machine and the staining looks good with two separate populations of CD45 RO positive and negative cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on FITC Channel

Results Summary

The results show a clear CD45RO positive and negative population, which confirms the specificity of the antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good Specificity

The Bad

None

The Bottom Line

Excellent antibody for identifying CD45 RO positive cells

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