Rabbit Antibody Against pY397 FAK

July 06, 2016

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Assay Biotech

Product Name:

Rabbit antibody against pY397 FAK

Catalog Number:

P12-1103

Browse Similar Products: pY397 Western Blot Antibodies

The rabbit antibody against pY397 FAK P12-1103 produced too many non-specific bands without any indication of phospo FAK induction.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tumor cell lysate

Primary Incubation

Overnight at 8-10 degrees Celsius, with rocking, 1 ug/ml in 5% BSA/PBS/0.05%Tween

Blocking Agent

5% BSA in PBS/0.05% Tween 20

Secondary Incubation

Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% BSA/PBS/Tw

Tertiary Incubation

None

Detection

Chemiluminescence: West Pico from Thermo Scientific

Results Summary

The antibody P12-1103 against pY397 FAK did not work in our hands.

Additional Notes

None

Related Categories

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Summary

The Good

None detected

The Bad

Too many non-specific bands

The Bottom Line

I would not recommend this antibody for cell signaling research.

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