Good T-Reg Marker

Department of Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Tonbo

Product Name:

CD25 APC

Catalog Number:

20-0259

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human prostate cells

Primary Incubation

Recommended for 20-30mins

Blocking Agent

FcX

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometer

Results Summary

CD25 cells +ve on T-Regs

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clear separation between +ve and -ve

The Bad

Not the best brightness

The Bottom Line

Very good for T-Reg identification

Share your experience with other scientists. Write a Review! »

Join the discussion