Santa Cruz
Beta-actin antibody
sc-47778
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Browse Similar Products: beta-Actin Western Blot Antibodies
Goal of the experiment was to determine the beta actin levels in different animals (rats). It was expected to be same as it is going to be used as a reference protein.
Western Blot
Rat retina
1:1000 overnight at 4 degrees celcius
5% dry milk powder
1:500 2 hrs at RT
Not applicable
Results show that the beta-actin levels found in all the tested samples were exactly equal as expected. However, it also gave one nonspecific band at a higher molecular weight.
None
Proper detection of the protein of interest.
Non specific binding.
It is good to use if slight non specific binding is not the issue.