Time Course Effect of Chemoattractant on Par-1 Expression

June 14, 2016

BlueskyReddit
Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

Thrombin R Antibody

Catalog Number:

Sc-5605

Protease activated receptors are belongs to G protein family. It is activated by action of serine protease such as thrombin. Here we checked the time course effect of chemoattractant on Par1 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5% BSA prepared in 1x TBST

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found Par1 showed time dependent increase by chemoattractant.

Additional Notes

None

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Summary

The Good

This antibody is very good for immunoprecipitation and western blot

The Bad

We have to use atleast 1:500 concentration to detect the band prominently

The Bottom Line

Thrombin exerts its effects via Pars. Among 4 types of Pars this Par1 is highly sensitive to thrombin. This antibody is very good for immunoprecipitaion and western blot.Highly recommended.

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