Santa Cruz Biotechnology
anti-NFkb p65
sc-372
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This antibody was used for comparing modulation of NFkb in different models of PD.
Western Blot
Rat brain homogenates
1:1000, Over night, 4˚C, incubated in 5% slime milk in TBS-T
5% slime milk in TBS-T
Goat anti-Rabbit IgG-HRP 1:4000, 1 hour, room temperature, incubated in 5% slime milk in TBS-T
Not necessary
Chemiluminescent detection
The antibody recognized various bands and gives a little background. One of the bands is at 66 KDa, which is the expected molecular weight.
None
Easy for standardization
It gives a little background.
It's good but is a little bit nonspecific.