Anti-BrdU Antibody

June 10, 2016

Pathology
Federal University of Paraná
Student

Overall

Quality of Results

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Company:

GE Healthcare

Product Name:

Anti-BrdU Antibody

Catalog Number:

RPN202

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To analyze proliferation, 70% confluent culture of astrocytes were labeled with 5uM BrdU and revealed with anti-BrdU antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat astrocyte purified culture

Primary Incubation

1:100, 45 minutes at room temperature

Blocking Agent

2% BSA

Secondary Incubation

1:500, 90 minutes, Room Temperature, in PBS

Tertiary Incubation

Counter-staining for 5 minutes with 1ug/ml DAPI

Detection

Fluorescence (FITC)

Results Summary

The antibody works really good,

Additional Notes

Cells were fixed with ice-cold Methanol, and treated 15 minutes with 2N HCl

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Summary

The Good

Excellent result

The Bad

Nothing

The Bottom Line

Good and reliable result in a short period of time

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