Santa Cruz Biotechnology
anti-IkB
sc-371
Info: View Product View Product Specs
Browse Similar Products: IkappaB-alpha Western Blot Antibodies
We were trying to analyze different levels of IkappaB in a model of Parkinson's Disease, as a way to measure activation of NF-kb
Western Blot
Brain protein extract
1:500, Over night, 4˚C, incubated in 5% slime milk in TBS-T
5% slime milk in TBS-T
1:4000, 1 hour, room temperature, incubated in 5% slime milk in TBS-T
Not necessary
Chemiluminescent
The antibody works well, as a poly-antibody it recognizes proteins of different molecular weights. It appears 2 different bands in the expected molecular weight range. It is not the best, but I believe it is worth the price.
None
Its gives a good signal with a low quantity of protein loading.
It gives more than 1 band.
It is worth the price.