Total RNA & MicroRNA Extraction

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

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Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNeasy Plus Mini Kit

Catalog Number:

74136

The aim of this experiment was to extract total RNA as well as the microRNA population from cultured cells.

Experimental Design and Results Summary

Application

Nucleic acid extraction method

Starting Material

Cultured cells

Protocol Overview

There are several appendices in the handbook that permit the kit to have various applications. In this experiment both the total RNA and microRNA population were extracted using the kit.

Tips

Read the appendices- very informative.

Results Summary

High yield and good purity of total RNA extracts as well as microRNA.

Additional Notes

None

Image Gallery

Summary

The Good

The same kit can be used for multiple applications.

The Bad

None

The Bottom Line

Very time efficient and good quality total RNA extracts.

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