Abcam
Anti-p38 antibody
ab27986
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Mouse pancreatic endothelial cells were growth arrested, treated with specific growth factor, protein extracts were made and resolved in 10% SDS PAGE. Resolved proteins were western transferred in nitrocellulose membrane and immunodetected with pp38 MAPK levels and same blot was reprobed with anti-p38 antibody, obtained from abcam, for normalization. Selection of antibody was because it does not cross react with JNK/SAPK or p42/44.
Western Blot
Mouse pancreatic endothelial cell extract
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscent detection in X-Ray film
Anti-p38 antibody from Abcam has detected only one specific band of p38 protein at correct size. The bend was well bright and highly specific.
Gives highly specific single band
Excellent antibody