Eppendorf Concentrator Plus

May 26, 2016

VU University Medical Center
Medical Oncology
Research Technician

Overall

Performance

Ease-of-Optimization

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Company:

Eppendorf

Product Name:

Concentrator plus

Catalog Number:

5305 000.304

Great concentrator, extremely easy to use and foolproof.

Experimental Design and Results Summary

Application

Concentrating samples

Starting Material

Peptides in Formic acid/acetonitrile

Protocol Overview

For over 10 years we have been concentrating samples using an old 3 way setup: a ventilated/heated centrifuge, a cold-trap and a pump. It was circumlocutory to use and requires a lot of space. The Eppendorf concentrator plus is a benchtop vacuumcentrifuge that is very easy to use and requires almost no setup. A timer can be set for the time required to dry and it will stop spinning after the time has passed, it will continue to heat though. It has 4 temperature settings: the (current) RT, 30°C, 45°C and 60°C. The brake can set to on/off. It also has several different functions which regard the vacuum to be applied. The strongest vacuum will be V-AQ(aqua solution) followed by V-AL(alcoholics), and V-HV(high vapor), these 3 functions will spin the rotor and turn on the vacuum. The concentrator plus can also act as a desiccator with the same settings without centrifuging. Finally it can also be used as a normal centrifuge, however the speed cannot be set.

Tips

Do not fill plates for more than 2/3 of the volume as stated by Eppendorf.

Results Summary

It takes roughly 1h and 45min to concentrate 48 eppendorf tubes from ~500µl to dry.

Features Summary

Extremely easy to use and foolproof.

Additional Notes

Be careful with overfilling plates.

Image Gallery

Summary

The Good

Easy to use, foolproof, benchtop(size).

The Bad

Only 4 temperature settings, speed cannot be regulated, plates can only be used for 2/3 of the volume.

The Bottom Line

It's a great benchtop concentrator (vacuumcentrifuge). Easy to setup out of the box (5 mins) and works like a charm. Plates could be a bit better though.

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