Astrocyte Imunohistochemistry

Pathology
Federal University of Paraná
Student

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Quality of Results

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Company:

DAKO

Product Name:

Antibody anti-GFAP

Catalog Number:

Z0334

Browse Similar Products: GFAP Immunofluorescence Antibodies

Primary culture astrocytes were tested under different stressing conditions and immunostained against GFAP to analyze phenotypic variation, a hallmark of reactive astrocytes in neurodegenertion.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat primary cortical astrocytes

Primary Incubation

1:700, Over night, 4˚C, incubated in PBS

Blocking Agent

5% BSA

Secondary Incubation

1:500, 90 minutes, Room Temperature, in PBS

Tertiary Incubation

NA

Detection

Fluorescence (FITC)

Results Summary

Primary antibody was very helpful to analyze purity of astrocytes in primary culture.

Additional Notes

None

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Summary

The Good

It never fails

The Bad

None

The Bottom Line

Excellent product!

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